Journal: Arthritis Research & Therapy

Article Title: Traditional Chinese medicine formula Bi-Qi capsule alleviates rheumatoid arthritis-induced inflammation, synovial hyperplasia, and cartilage destruction in rats

doi: 10.1186/s13075-018-1547-6

Figure Lengend Snippet: Collagen-induced arthritis (CIA) enhanced osteopontin (OPN) and cartilage oligomeric matrix protein (COMP) expression in paw joint cartilage, while the Bi-Qi capsule or methotrexate (MTX) treatment reduced this effect. Representative immunohistochemistry images of paw joint synovium with OPN and COMP immunostaining

Article Snippet: After blocking of non-specific binding sites with 10% normal goat serum for 1 h, the sections were incubated overnight at 4 °C with mouse-anti-COMP antibody (Boiss antibodies company, Beijing, China) in PBS or mouse anti-OPN antibody (Boster Bioengineering Co.Ltd., Wuhan, China) in PBS.

Techniques: Expressing, Immunohistochemistry, Immunostaining

Journal: Arthritis Research & Therapy

Article Title: Traditional Chinese medicine formula Bi-Qi capsule alleviates rheumatoid arthritis-induced inflammation, synovial hyperplasia, and cartilage destruction in rats

doi: 10.1186/s13075-018-1547-6

Figure Lengend Snippet: Bi-Qi capsule (BQ) or methotrexate (MTX) reduced collagen-induced arthritis (CIA)-induced osteopontin (OPN) and cartilage oligomeric matrix protein (COMP) upregulation in serum, and mRNA and protein levels in paw joint tissue. a, b OPN and COMP levels in serum. c, d OPN and COMP mRNA expression in paw joint cartilage. e, f OPN and COMP protein quantification from immunohistochemistry images of paw joint synovium. Data are presented as mean ± SD from eight rats in each group. Significant effect of treatment compared to control: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 . Significant effect of treatment compared to arthritic group: #### P < 0.0001 . Significant effect of treatment compared to high-dose BQ (BQ-HD): $$ P < 0.01, $$$$ P < 0.0001 . Significant effect of treatment compared to MTX-group: & P < 0.05, &&&& P < 0.0001. BQ-MD, moderate-dose BQ

Article Snippet: After blocking of non-specific binding sites with 10% normal goat serum for 1 h, the sections were incubated overnight at 4 °C with mouse-anti-COMP antibody (Boiss antibodies company, Beijing, China) in PBS or mouse anti-OPN antibody (Boster Bioengineering Co.Ltd., Wuhan, China) in PBS.

Techniques: Expressing, Immunohistochemistry, Control

Journal: PLoS ONE

Article Title: Effect of CGRP-Adenoviral Vector Transduction on the Osteoblastic Differentiation of Rat Adipose-Derived Stem Cells

doi: 10.1371/journal.pone.0072738

Figure Lengend Snippet: Primers used for real-time PCR analysis.

Article Snippet: Following transfer, membranes were blocked with a solution of 0.1% Tween 20/TBS (TBS/T) containing 5% non-fat milk for one hour at room temperature and then incubated overnight at 4°C with monoclonal mouse anti-human CGRP and OPN antibodies (GeneTex, USA, final dilution 1∶300) or rabbit polyclonal anti-human Collagen I and BPG antibodies (Chemicon, Temecula, California, USA, final dilution 1∶400).

Techniques: Real-time Polymerase Chain Reaction

Journal: PLoS ONE

Article Title: Effect of CGRP-Adenoviral Vector Transduction on the Osteoblastic Differentiation of Rat Adipose-Derived Stem Cells

doi: 10.1371/journal.pone.0072738

Figure Lengend Snippet: A) CGRP-transduced ADSCs exhibited bright green EGFP fluorescence under fluorescent microscope and indicated a transduction efficiency of approximately 81%. Scale bar equals 100 µm. B) The growth curves of different groups showed that the transduced group had a significantly shorter doubling time than the untransduced group. *p <0.05, transduced group vs. control group.

Article Snippet: Following transfer, membranes were blocked with a solution of 0.1% Tween 20/TBS (TBS/T) containing 5% non-fat milk for one hour at room temperature and then incubated overnight at 4°C with monoclonal mouse anti-human CGRP and OPN antibodies (GeneTex, USA, final dilution 1∶300) or rabbit polyclonal anti-human Collagen I and BPG antibodies (Chemicon, Temecula, California, USA, final dilution 1∶400).

Techniques: Fluorescence, Microscopy, Transduction, Control

Journal: PLoS ONE

Article Title: Effect of CGRP-Adenoviral Vector Transduction on the Osteoblastic Differentiation of Rat Adipose-Derived Stem Cells

doi: 10.1371/journal.pone.0072738

Figure Lengend Snippet: A), B) demonstrated higher expression of CGRP in the transduced group than the control group at 3 d or 7 d after transduction by RT-PCR and Western blot detections. *p <0.05, transduced group vs. control group.

Article Snippet: Following transfer, membranes were blocked with a solution of 0.1% Tween 20/TBS (TBS/T) containing 5% non-fat milk for one hour at room temperature and then incubated overnight at 4°C with monoclonal mouse anti-human CGRP and OPN antibodies (GeneTex, USA, final dilution 1∶300) or rabbit polyclonal anti-human Collagen I and BPG antibodies (Chemicon, Temecula, California, USA, final dilution 1∶400).

Techniques: Expressing, Control, Transduction, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: PLoS ONE

Article Title: Effect of CGRP-Adenoviral Vector Transduction on the Osteoblastic Differentiation of Rat Adipose-Derived Stem Cells

doi: 10.1371/journal.pone.0072738

Figure Lengend Snippet: A), B) Expression of the CGRP gene in the transduced group showed higher secretion of CGRP protein than in the control group after 3days and 7days. Scale bar equals 100 µm. *p <0.05, transduced group vs. control group.

Article Snippet: Following transfer, membranes were blocked with a solution of 0.1% Tween 20/TBS (TBS/T) containing 5% non-fat milk for one hour at room temperature and then incubated overnight at 4°C with monoclonal mouse anti-human CGRP and OPN antibodies (GeneTex, USA, final dilution 1∶300) or rabbit polyclonal anti-human Collagen I and BPG antibodies (Chemicon, Temecula, California, USA, final dilution 1∶400).

Techniques: Expressing, Control

Journal: PLoS ONE

Article Title: Effect of CGRP-Adenoviral Vector Transduction on the Osteoblastic Differentiation of Rat Adipose-Derived Stem Cells

doi: 10.1371/journal.pone.0072738

Figure Lengend Snippet: A) X-rays photograph of rat radical defects in different groups at 2 weeks, 4 weeks, and 12 weeks showing complete bridging of the defect in group 3, however, a radiolucent line was still present at the defect site in group 1 at 12 weeks. B) Radiographical scoring also showed that new bone formation in group 3 was more significant difference than the other groups at 12 weeks (p<0.05). (Group 1 referred to β -TCP group; Group 2 referred to ADSCs/ β -TC group; Group 3 referred to Ad-CGRP-ADSCs/ β -TCP group).

Article Snippet: Following transfer, membranes were blocked with a solution of 0.1% Tween 20/TBS (TBS/T) containing 5% non-fat milk for one hour at room temperature and then incubated overnight at 4°C with monoclonal mouse anti-human CGRP and OPN antibodies (GeneTex, USA, final dilution 1∶300) or rabbit polyclonal anti-human Collagen I and BPG antibodies (Chemicon, Temecula, California, USA, final dilution 1∶400).

Techniques:

Journal: PLoS ONE

Article Title: Effect of CGRP-Adenoviral Vector Transduction on the Osteoblastic Differentiation of Rat Adipose-Derived Stem Cells

doi: 10.1371/journal.pone.0072738

Figure Lengend Snippet: HE staining of the transverse bone defect sections in different groups at 4 weeks and 12 weeks post-surgery. Abbreviations used: scaffold (S); new bone from implanted scaffold (NB-S); bone marrow (BM); cortical bone (CB), trabecular bone (TB). Scale bar equals 400µm. (Group 1 referred to β -TCP group; Group 2 referred to ADSCs/ β -TC group; Group 3 referred to Ad-CGRP-ADSCs/ β -TCP group).

Article Snippet: Following transfer, membranes were blocked with a solution of 0.1% Tween 20/TBS (TBS/T) containing 5% non-fat milk for one hour at room temperature and then incubated overnight at 4°C with monoclonal mouse anti-human CGRP and OPN antibodies (GeneTex, USA, final dilution 1∶300) or rabbit polyclonal anti-human Collagen I and BPG antibodies (Chemicon, Temecula, California, USA, final dilution 1∶400).

Techniques: Staining

Journal:

Article Title: Elevated plasma osteopontin associated with gastric cancer development, invasion and survival

doi: 10.1136/gut.2006.109868

Figure Lengend Snippet: Figure 2 Relative quantitation of osteopontin (OPN) expression in gastric cancer tissues. A–D, Immunolocalisation of OPN in human gastric tissues. OPN expression is indicated by brown staining and is immunolocalised using anti‐OPN monoclonal antibody and avidin–biotin peroxidase complex and method with diaminobenzidine as the chromogen. The sections were counterstained with haematoxylin. A, Healthy gastric epithelial cells with no expression of OPN. B, Epithelial cells of atrophic gastritis with weak expression of OPN. C and D, Gastric adenocarcinoma with OPN expression in cancer cells. Magnification: A, B ×250; C ×400; D ×600.

Article Snippet: Sections were then incubated with mouse anti‐OPN monoclonal antibodies (Novocastra Laboratories Ltd, Newcastle upon Tyne, UK) at a dilution of 1:50 overnight at 4 °C, and washed twice in PBS, before being incubated in appropriate biotinylated secondary antibody for 10 min.

Techniques: Quantitation Assay, Expressing, Staining, Avidin-Biotin Assay